Coding
Sticky fli

Part:BBa_K2845001:Design

Designed by: Oliver Baltensperger, Antonio Martínez Brotons   Group: iGEM18_ETH_Zurich   (2018-10-10)


sticky fliC (BBa_K2845000) with promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 792
    Illegal BamHI site found at 1127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 357
    Illegal AgeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sticky fliC (BBa_K2845000) under the promoter J23104 and RBS B0032 should result in the expression of the sticky FliC flagellar protein. It is a truncated version of the normal fliC, but sticks to surfaces like glass or polystyrene. The motility function should be maintained compared to the normal protein. All of these features still lack more in-depth characterization.